Application

Research CategoryApoptosis & Cancer

Detect SOX9 using this mouse monoclonal Anti-SOX9, clone 4B7.1 Antibody, Cat. No. MABC785, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SOX9 in human liver cancer and small intestine tissue sections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Transcription factor SOX-9 (UniProt P48436; also known as Sex-determining region Y-box 9 protein, SRY-related HMG-box gene 9) is encoded by the SOX9 (also known as CMD1, CMPD1, SRA1) gene (Gene ID 6662) in human. Sox family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences encoding for the HMG-box domains that mediate sequ+D11ence-specific DNA binding. SOX genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at at least 40 genomic loci and at least 30 Sox genes have been identified. SOX-9 is a key regulator of developmental processes, including male sex determination, chondrogenesis, neurogenesis, and neural crest development. SOX-9 up-regulation is reported in cancers of various origins, including breast, colon, prostate, and lung, where its expression correlates with malignancy and patient survival. Cellular SOX9 mRNA is shown to be targeted and negatively regulated+D11 by miR-32, and the cellular miR-32 level is reported to be significantly lower in non-small cell lung cancer (NSCLC) compared with noncancerous lung tissues.

Immunogen

KLH-conjugated linear peptide corresponding to human SOX9 C-terminal end sequence.

Linkage

Replaces: MABC672

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G purified.

Format: Purified

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SOX9 in 10 µg of HepG2 cell lysate.

Specificity

Clone 4B7.1 epitope lies within the highly conserved C-terminal end region of SOX9.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~69 kDa observed. 54.85/56.14/56.08/56.12/56.12 kDa (chicken/human/mouse/porcine/rat) calculated. Uncharacterized bands may be observed in some lysate(s).